The Dons were in command following first-half goals from Aaron Wilbraham and James Tunnicliffe before Daniel Ward netted a late consolation for the luckless Robins, who hit the woodwork three times.
Gleeson missed the 1-0 win over Leyton Orient on Saturday after injuring his ankle in training on Friday and is a doubt to be fit for Paul Ince's side.
The game between Milton Keynes Dons and Swindon Town in the League One on Tuesday, 9th March 2010 at 19:45 takes centre stage for FootyMad's preview analysis.
Mark Carrington headed in the only goal of the game after 31 minutes as Ince's men kept their slim play-off hopes alive by moving to within seven points of the top six.
Midfielder Carrington registered the only goal of the game in the 31st minute although the Dons missed a host of other chances as they cruised to victory over a lacklustre Os side.
The game between Milton Keynes Dons and Leyton Orient in the League One on Saturday, 6th March 2010 at 15:00 takes centre stage for FootyMad's preview analysis.
O'Hanlon is working his way back to fitness having been sidelined for the last eight months with a cruciate knee ligament injury, making his first substitute appearance in last week's game against Yeovil.
A host of football superstars will tee off at the "Golf Champions of Football" in Portugal this June in support of 'Keep Your Eye on the Ball', an initiative run by The FA.